Effect of Genistein Analogs on Oxygen Radical Production in Leukocytes Stimulated by Unopsonized Zymosan

Jieun Yun; Sei Ryang Oh; Hyeong-Kyu Lee; IL-Moo Chang; Kiyoshi Takatsu; Yeo-Pyo Yun; Kyung Rak Min; Youngsoo Kim

doi:10.1055/s-2001-12002

Planta Med 2001; 67(3): 274-276
DOI: 10.1055/s-2001-12002

Letter

Effect of Genistein Analogs on Oxygen Radical Production in Leukocytes Stimulated by Unopsonized Zymosan

Jieun Yun¹ , Sei Ryang Oh² , Hyeong-Kyu Lee² , IL-Moo Chang³ , Kiyoshi Takatsu⁴ , Yeo-Pyo Yun¹ , Kyung Rak Min¹ , Youngsoo Kim^1,*

¹ College of Pharmacy, Chungbuk National University, Cheongju, Korea
² Korea Research Institute of Bioscience & Biotechnology, Taejon, Korea
³ Natural Products Research Institute, Seoul National University, Seoul, Korea
⁴ Institute of Medical Science, University of Tokyo, Tokyo, Japan

Abstract

Effects of genistein analogs on oxygen radical production have been analyzed in human neutrophils, human monocytes or murine macrophages Raw264.7 stimulated with unopsonized zymosan by lucigenin- and luminol-enhanced chemiluminescence assays. Genistein exhibited IC₅₀ values of 10.7 - 11.5 μM on the oxygen radical production in human neutrophils, 10.9 - 11.0 μM in human monocytes, and 14.8 - 27.3 μM in Raw264.7 cells. Orobol, a genistein analog with an additional hydroxy group at the 3′ position, exhibited IC₅₀ values of 3.0 - 3.3 μM on the oxygen radical production in human neutrophils, 2.8 - 3.1 μM in human monocytes, and 1.5 - 3.9 μM in Raw264.7 cells. Genistin and sophoricoside are genistein glycosides with a glucose moiety at 7 or 4′ position, respectively. The genistein glycosides exhibited 23 - 37 % inhibitory effects at 100 μM on the oxygen radical production.

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Prof. Youngsoo Kim, Ph.D.

College of Pharmacy

Chungbuk National University

Cheongju 361-763

Republic of Korea

Email: youngsoo@cbucc.chungbuk.ac.kr

Fax: +82-43-268-2732